Enzyme concentration coursework

However, there are some factors that I must take into consideration. Choice of Method I tried to select the method I considered would be most accurate. However, equipment for rapidly mixing liquids allows fast kinetic measurements on initial rates of less than one second. Also, I noticed if the barrel was wet, the syringe often got stuck for a short time before it recorded the volumes of gas.

Thus, Km is the substrate concentration value in which the substrate concentration is reaching halfway of the maximum reaction velocity. The line of best fit also suggests values of concentrations which I have not investigated.

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This means that in my results, the volume of gas produced in the first 5 seconds may have been higher than it should have been if I had Enzyme concentration coursework exactly 0. Though the enzymatic mechanism for the unimolecular reaction ES. I believe that the data also shows strong positive correlation, and there are few outliers, which shows that my results are accurate.

The higher the concentration, the more heat will be produced. Firstly, more oxygen dissolves in water at low temperatures than at high temperatures, meaning that for the reactions involving low concentrations, more oxygen would have dissolved than in the higher concentrations because of the decreased amount of heat energy given off.

Enzyme kinetics

The slope in the initial rate period is the initial rate of reaction v. I tried to make this as accurate as possible by keeping my eyes level with the gas syringe. Here, the rate of reaction becomes dependent on the ES complex and the reaction becomes a unimolecular reaction with an order of zero.

Catalase is an example of this, as the enzyme reacts with a first molecule of hydrogen peroxide substrate, becomes oxidised and is then reduced by a second molecule of substrate. If there were fewer molecules of hydrogen peroxide, there would have been fewer collisions between molecules of enzyme and substrate, resulting in fewer enzyme-substrate complexes being made.

Overall, there is a pattern showing a constant trend in that as the concentration decreases the rate of reaction decreases too, and that the overall volume of gas evolved also decreases. Surface Area of Yeast Molecules I ground up the yeast to try to make the surface area as similar as possible because surface area is a major factor in my experiment.

This results in a bigger proportion of molecules having a kinetic energy greater than that of the activation energy. There is an initial bimolecular reaction between the enzyme E and substrate S to form the enzyme—substrate complex ES.

I believed this was accurate, but upon reflection, using a pipette would have been much more accurate as pipettes have a much lower apparatus error than volumetric flasks.volume-'; (c) enzyme concentration was determined by dividing the Vm,x value from (b) by the turnover number from (a). The total enzyme site concentration was finally determined by multiplying enzyme concentration by the number of subunits per holoenzyme, assuming one active site per subunit.

Effect of Substrate Concentration on the Rate of Activity of Catalase

Substrate concentration is the amount of substrate present that can be turned into product and is most commonly measured in molarity (moles per liter). The concentration of substrates is often used to measure enzyme activity, which is based on the rate of a reaction (product formed over time).

Jan 29,  · As Level Biology Coursework Effect of enzyme concentration, pectinex on apple juice production? Have completed experiment and collected results but my results dont seem to be very conclusive.

Anyone have any results or advice on what i should be seeing- more precise than just enzyme concentration increases, apple Status: Resolved. The aim: Determination of the effect of enzyme concentration on catalysis using starch an amylase.

Enzymes are said to be catalytic proteins which. Apr 30,  · The curve will eventually plateau when the enzyme molecules have become fully saturated. This is called the maximum velocity of the reaction or Vmax.

The substrate concentration at this point, even if increased, will not affect the rate of reaction because it is the enzyme which is in low mint-body.coms: 5. For a given enzyme concentration and for relatively low substrate concentrations, the reaction rate increases linearly with substrate concentration; the enzyme molecules are largely free to catalyse the reaction, and increasing substrate concentration means an increasing rate at which the enzyme and substrate molecules encounter one another.

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Enzyme concentration coursework
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